MS/MS ALL with DIA-PASEF Acquisition Comprehensive Quantification

Data-independent acquisition (dia)-PASEF^® is both more sensitive and selective than traditional DIA-MS approaches as it applies the PASEF^® principle to combine the advantages of DIA with the inherent ion efficiency of PASEF^®. Over the entire liquid chromatography-mass spectrometry (LC-MS)/MS dia-PASEF^® run, a perfect data cuboid is created containing m/z, ion mobility (CCS), retention time and intensity. TIMS separation increases selectivity, excludes singly charged precursors from fragmentation and cleans up the sample by concentrating signals from noise. Making use of the correlation of molecular weight and CCS coded information from the dual-TIMS funnel, dia-PASEF^® enables highly confident identification.