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Biomolecular and Proteomics Mass Spectrometry Facility BPMSF
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Instrumentation: TimsTOF HT (QTOF)

The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF® acquisition modes including PASEF®, dia-PASEF® and prm-PASEF® achieving flexibility in 4D-Proteomics™. Simply uncompromised proteomic depth at scale.

The TimsTOF HT is an innovative breakthrough in LC-MS2 performance that uniquely integrates comprehensive qualitative exploration, rapid profiling, and high-resolution quantitation workflows on a single platform. It combines the highest sensitivity detection, high-resolution with at least 5X better acquisition speed, and stable ~1ppm mass accuracy over days of acquisition.

  • TIMS is first and foremost a separation technique in the gas phase. This resolves sample complexity through an added dimension of separation in addition to high performance liquid chromatography (HPLC) and mass spectrometry, increasing peak capacity and confidence in compound characterization.

    Equally important, the TIMS device also accumulates and concentrates ions of a given m/z and mobility, enabling a unique increase in sensitivity and speed.

    A near 100% duty cycle can be achieved with the dual-TIMS technology facilitating accumulation in the front section, while ions in the rear section are sequentially released depending on their mobility. This process of parallel accumulation serial fragmentation (PASEF®) enables collisional cross section (CCS) analysis.