Instrumentation: TimsTOF 2 Pro (QTOF)

The timsTOF Pro 2, powered by the latest parallel accumulation serial fragmentation (PASEF^®) technology, paves the way for 4D-Multiomics applications. With its proven robustness, high sensitivity and fast MS/MS acquisition, timsTOF Pro 2 is the platform of choice for 4D-Proteomics.

TimsTOF 2 Pro is coupled to high throughput EvoSep Ultra-HPLC systems for LC-MSMS analysis. For specifications on these instruments please follow the links to the manufacturer's websites.

The TimsTOF 2 Pro is an innovative breakthrough in LC-MS2 performance that uniquely integrates comprehensive qualitative exploration, rapid profiling, and high-resolution quantitation workflows on a single platform. It combines the highest sensitivity detection, high-resolution with at least 5X better acquisition speed, and stable ~1ppm mass accuracy over days of acquisition.

• TIMS is first and foremost a separation technique in the gas phase. This resolves sample complexity through an added dimension of separation in addition to high performance liquid chromatography (HPLC) and mass spectrometry, increasing peak capacity and confidence in compound characterization.
  
  Equally important, the TIMS device also accumulates and concentrates ions of a given m/z and mobility, enabling a unique increase in sensitivity and speed.
  
  A near 100% duty cycle can be achieved with the dual-TIMS technology facilitating accumulation in the front section, while ions in the rear section are sequentially released depending on their mobility. This process of parallel accumulation serial fragmentation (PASEF^®) enables collisional cross section (CCS) analysis.