Instrumentation
- HDX MS
- TimsTOF 2 Pro
- Orbitrap Lumos FAIMS with ETD
- SCP TimsTOF
The timsTOF SCP for quantitative single cell biology research with unbiased, deep single-cell 4D-Proteomics™, immunopeptidomics, epiproteomics and PTM analysis to complement scRNA-seq. Expanding the horizons of single cell research.
timsTOF SCP is coupled to high throughput EvoSep Ultra-HPLC systems for LC-MSMS analysis. For specifications on these instruments please follow the links to the manufacturer's websites.
Advanced ion optics and PASEF® to investigate cell heterogeneity and biology from a single cell
Mass spectrometric proteomics has become a staple of modern research in understanding biological function and disease mechanisms. Healthy or diseased tissues that seem homogenous are composed of cells with a variety of different proteomes. The challenge of deciphering the proteomes in each single cell – the cell heterogeneity – holds the key to fully understanding its function.
The timsTOF SCP offers a radically improved ion source concept. Combined with parallel accumulation serial fragmentation (PASEF®) acquisition methods, it provides extremely high speed and sensitivity to tackle proteomes of single cells or post translational modifications in a few cells that are morphologically or functionally similar.
The facility is supported by the NIH shared instrumentation grant numbers
S10 OD016234 (Synapt-HDX-MS) and S10 OD021724 (LUMOS Orbi-Trap).
Please reference these grant numbers for publications resulting from data obtained from these instrumentations.
Funds from Moores Cancer Center of UC San Diego