Post Translational Modifications (PTM)

Post translational modifications are important in biological processes and mass spectrometers are becoming the instruments of choice for the detection and characterization of PTMs. Essentially mass spectrometers have the capability to characterize most fixed mass modification that are stable in proteins. These include protein acetylation, ubiquitination, methylation and phosphorylation. The most important consideration in characterizing PTMs is their frequency of occurrence. Since most PTMs are rare events specific biochemical enrichment strategies need to be used to facilitate their identification and characterization. For example TiO2 and metal based resins (IMAC) are commonly used for the enrichment of phospho peptides before mass spectrometry analysis. PTM based project should be well researched and discussed to achieve the best outcome.

Supported By

The facility is supported by the NIH shared instrumentation grant numbers

S10 OD016234 (Synapt-HDX-MS) and  S10 OD021724 (LUMOS Orbi-Trap).

Please reference these grant numbers for publications resulting from data obtained from these instrumentations.

Funds from Moores Cancer Center of UC San Diego